Thermo Fisher Scientific RRAS Monoclonal Antibody (2E12)

Applications and Tested Dilution

Product Specifications

Product Specific Information

Sequence of this protein is as follows:
AINDRQSFNE VGKLFTQILR VKDRDDFPVV LVGNKADLES QRQVPRSEAS AFGASHHVAY FEASAKLRLN VDEAFEQLVR AVRKYQEQEL PPSPPSAPRK KGGGCPCVLL

Target Information

The Ras superfamily of small GTPases includes the Ras, Rho, Arf, Rab, and Ran families. Ras-regulated signal pathways control processes such as actin cytoskeletal integrity, proliferation, differentiation, cell adhesion, apoptosis, and cell migration. These GTPases function as molecular switches that control eukaryotic cell function by cycling between two interconvertible forms: a GDP-bound inactive form and a GTP-bound active form. The binding of GTP induces a conformational change in the effector-binding domain of the G-protein.

Small GTPases are monomeric G-proteins with molecular masses of approximately 20–30 kDa. Ras is membrane-associated through prenylation. Mutations in Ras family proto-oncogenes (H-Ras, N-Ras, K-Ras) are among the most common in human tumors (20–30% of all cases).

• K-Ras mutations: non-small-cell lung, colorectal, pancreatic carcinomas
• H-Ras mutations: bladder, kidney, thyroid carcinomas
• N-Ras mutations: melanoma, hepatocellular carcinoma, hematologic malignancies

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.