Thermo Fisher Scientific Horse anti-Goat IgG (H+L) ReadyProbes Secondary Antibody, Biotin

Applications

Tested Dilution

Western Blot (WB)

Assay-dependent

Immunohistochemistry (IHC)

Assay-dependent

Immunocytochemistry (ICC/IF)

Assay-dependent

ELISA (ELISA)

Assay-dependent

In Situ Hybridization (ISH)

Assay-dependent

Product Specifications

Species Reactivity

Goat

Host/Isotype

Horse / IgG

Class

Polyclonal

Type

Secondary Antibody

Conjugate

Biotin Biotin Biotin

Form

Liquid

Concentration

10 µg/mL

Purification

Affinity chromatography

Storage buffer

PBS with carrier protein

Contains

0.08% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

Target

IgG

Antibody Form

Whole Antibody

Product Specific Information

Recognizes both heavy and light chains (H+L). Suitable for use with goat, sheep, and bovine IgG primary antibodies

No pipetting or dilutions required. Just tip and drip enough reagent to adequately cover the tissue or cells (typically 2-3 drops).

Target Information

A Horse anti-Goat IgG antibody is an immunoglobulin produced by immunizing a horse with goat IgG. This generates a specific immune response in the horse, leading to the production of antibodies that can recognize and bind to goat IgG. In scientific assays, such as Western blotting, ELISA, or immunohistochemistry, this antibody is used as a secondary antibody to detect a goat-derived primary antibody bound to its target antigen. Typically, the horse anti-goat IgG antibody is conjugated to a reporter molecule (e.g., an enzyme like horseradish peroxidase or a fluorescent dye), which allows for the indirect detection and quantification of the antigen-antibody complex. This system amplifies the signal, making it easier to detect low-abundance targets.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.