Thermo Fisher Scientific DDIT3 Polyclonal Antibody, Biotin
DDIT3(CHOP/GADD153) 단백질을 인식하는 Biotin 결합 토끼 폴리클로날 항체. Western blot, ELISA, IP에 적합하며 사람, 생쥐, 랫트 시료에 반응. Affinity chromatography로 정제된 고품질 항체로 연구용으로 사용 가능.
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- GADD153-BIOTIN
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| ELISA | 1:10,000 |
| Immunoprecipitation (IP) | 1:50–1:250 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide taken within amino acid region 60–160 on human GADD153 protein |
| Conjugate | Biotin |
| Form | Liquid |
| Concentration | 0.5–1.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | Proprietary buffer, pH 7.4–7.8, with 30% glycerol, 0.5% BSA |
| Contains | 0.02% sodium azide |
| Storage Conditions | –20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
GADD153 (also known as DDIT3 or CHOP) is a small nuclear protein that can dimerize with transcription factors C/EBP alpha and beta. This dimerization inhibits the normal binding and function of C/EBP to classical binding sites, while enabling binding to non-classical C/EBP stress-related targets. Under normal cellular conditions, GADD153 expression is low, but it is highly upregulated during cellular or ER stress, such as treatment with tunicamycin, nutrient starvation, or reducing agents that affect ER calcium flux.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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