Thermo Fisher Scientific TJP1/ZO-1 Recombinant Rabbit Monoclonal Antibody (BLR092G)
TJP1/ZO-1 단백질을 인식하는 Thermo Fisher Scientific의 재조합 토끼 단클론 항체. Western blot, IHC, ICC/IF, IP 등 다양한 응용 가능. 인간 및 생쥐 반응성, 고순도 액상 형태, 4°C 보관. 세포 간 밀착 결합 연구에 적합.
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Thermo Fisher Scientific TJP1/ZO-1 Recombinant Rabbit Monoclonal Antibody (BLR092G)
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100 - 1:500 |
| Immunocytochemistry (ICC/IF) | 1:100 - 1:500 |
| Immunoprecipitation (IP) | Assay-dependent |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | BLR092G |
| Immunogen | Peptide representing a region between residue 650 and 700 of human tight junction protein ZO-1 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 500 µg/mL |
| Purification | Purified |
| Storage Buffer | BBS, pH 7.8–8.6, with 0.1% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Target Information
ZO-1 forms complexes with either ZO-2 or ZO-3. These proteins can associate with claudin, occludin, and F-actin at tight junction strands, providing a linkage between the actin cytoskeleton and tight junctions.
ZO-1 expression is significantly reduced in many breast cancer lines. ZO-2 and ZO-3 are ubiquitously expressed within epithelial tight junctions.
ZO-1 is located on the cytoplasmic membrane surface of intercellular tight junctions and may be involved in signal transduction at cell-cell junctions.
Two transcript variants encoding distinct isoforms have been identified for the ZO-1 gene.
Diseases associated with ZO-1 dysfunction include Celiac Disease and Congenital Nephrotic Syndrome.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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