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Thermo Fisher Scientific Rat anti-Mouse IgG1 Secondary Antibody, FITC, eBioscience
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Thermo Fisher Scientific Rat anti-Mouse IgG1 Secondary Antibody, FITC, eBioscience

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Rat monoclonal anti-Mouse IgG1 FITC-conjugated secondary antibody. Flow cytometry, ICC/IF, IHC(F/P)에 적합. 488 nm excitation, 520 nm emission. 높은 특이성과 민감도를 제공하며, 비동결 상태로 4°C 보관.

카탈로그번호
11-4015-8x (2개 옵션)
판매단위
pk
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마지막 업데이트 2025. 08. 02. 오후 01:22
Thermo Fisher Scientific 11-4015-82 Rat anti-Mouse IgG1 Secondary Antibody, FITC, eBioscience 100 ug pk판매 단위 pk ·
재고 확인 필요
254,200원VAT 포함 279,620원
Thermo Fisher Scientific 11-4015-80 Rat anti-Mouse IgG1 Secondary Antibody, FITC, eBioscience 25 ug pk판매 단위 pk ·
재고 확인 필요
106,600원VAT 포함 117,260원

Thermo Fisher Scientific · Thermo Fisher Scientific Rat anti-Mouse IgG1 Secondary Antibody, FITC, eBioscience

Applications and Tested Dilutions

Application Tested Dilution
Immunohistochemistry (Paraffin) (IHC (P)) Assay-Dependent
Immunohistochemistry (Frozen) (IHC (F)) Assay-Dependent
Immunocytochemistry (ICC/IF) Assay-Dependent
Flow Cytometry (Flow) 0.5 µg/test

Product Specifications

항목 내용
Species Reactivity Mouse
Host / Isotype Rat / IgG
Class Monoclonal
Type Secondary Antibody
Clone M1-14D12
Conjugate FITC
Excitation / Emission Max 498 / 517 nm
Form Liquid
Concentration 0.5 mg/mL
Purification Affinity chromatography
Storage Buffer PBS, pH 7.2
Contains 0.09% sodium azide
Storage Conditions 4°C, store in dark, DO NOT FREEZE
Shipping Conditions Ambient (domestic); Wet ice (international)
RRID AB_11044501
Target IgG1
Antibody Form Whole Antibody

Available Conjugate Formats

  • APC
  • Biotin
  • eFluor 450
  • eFluor 570
  • eFluor 615
  • eFluor 660
  • HRP
  • PE
  • PE-Cyanine7
  • PE-eFluor 610
  • PerCP-eFluor 710
  • Super Bright 436
  • Super Bright 600
  • Super Bright 780
  • Custom conjugation available upon request

Product Specific Information

The monoclonal antibody M1-14D12 recognizes Mouse IgG1 antibodies and can be used as a secondary reagent in flow cytometry and microscopy.
This antibody does not recognize other mouse isotypes or cross-react with rat IgG1 or other rat isotypes.

Applications Reported: Flow cytometry, immunocytochemistry, and immunohistochemistry (frozen and paraffin-embedded tissue).
Applications Tested: Tested by flow cytometric analysis of normal human peripheral blood cells stained with a mouse IgG1 primary antibody. Recommended ≤ 0.5 µg per test (100 µL final volume). Optimal titration required.

Excitation: 488 nm
Emission: 520 nm
Laser: Blue Laser
Filtration: 0.2 µm post-manufacturing filtered


Target Information

Anti-Mouse secondary antibodies are affinity-purified with high specificity for mouse immunoglobulins.
They are useful for detection, sorting, or purification of target proteins and enable versatile detection systems (HRP, AP, fluorescence).
Signal amplification increases sensitivity, as multiple secondary antibodies can bind to a single primary antibody.
Typically generated by immunizing host animals with pooled mouse immunoglobulins and purified via immunoaffinity chromatography or further modified for enhanced specificity.


For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.


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