Thermo Fisher Scientific Rat IgG2b kappa Isotype Control (eB149/10H5), FITC, eBioscience
FITC로 결합된 Rat IgG2b kappa 동형 대조항체로, 면역형광, 유세포분석, 면역조직화학 및 면역세포화학 실험에 적합합니다. 청색 레이저(488 nm)에서 여기되어 520 nm에서 방출하며, 실험 항체와 동일 농도로 사용 가능합니다. 연구용 전용 제품입니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Immunohistochemistry (IHC) | Assay-Dependent |
| Immunocytochemistry (ICC/IF) | Assay-Dependent |
| Flow Cytometry (Flow) | Assay-Dependent |
| Control (Ctrl) | Assay-Dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Host / Isotype | Rat / IgG2b, kappa |
| Class | Monoclonal |
| Type | Isotype Control |
| Clone | eB149/10H5 |
| Conjugate | FITC |
| Excitation / Emission Max | 498 / 517 nm |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_470003 |
Additional Formats Available
- Unconjugated
- Alexa Fluor 488
- Alexa Fluor 532
- Alexa Fluor 561
- Alexa Fluor 647
- Alexa Fluor 660
- Alexa Fluor 700
- APC
- APC-eFluor 780
- Biotin
- Brilliant UV 395 / 496 / 563 / 615 / 661 / 737 / 805
- Brilliant Violet 421 / 480 / 605 / 650 / 711 / 786
- eFluor 450 / 506 / 570 / 660
- Functional Grade
- PE / PE-Cyanine5 / PE-Cyanine5.5 / PE-Cyanine7 / PE-eFluor 610
- PerCP-Cyanine5.5 / PerCP-eFluor 710
- Super Bright 436 / 600 / 645 / 702 / 780
- Custom conjugation available upon request
Product Specific Information
The rat IgG2b, kappa monoclonal antibody serves as an isotype control immunoglobulin.
It has been reported for use in:
- Immunofluorescent staining
- Flow cytometric analysis
- Immunohistochemistry
- Immunocytochemistry
Applications Tested:
Validated by immunofluorescent staining and flow cytometric analysis of mouse splenocyte suspensions.
Use at the same concentration as the experimental antibody.
Fluorescence Characteristics:
- Excitation: 488 nm
- Emission: 520 nm
- Compatible Laser: Blue Laser
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
Rat IgG2b encodes the immunoglobulin G2b subclass of rat antibodies, a key component of the immune system responsible for pathogen neutralization and opsonization.
It is widely used in research applications such as immunohistochemistry, flow cytometry, and Western blotting.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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