Thermo Fisher Scientific MED12 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:2,000-1:10,000

-

Immunohistochemistry (Paraffin) (IHC (P))

1:1,000 to 1:5,000

-

Immunoprecipitation (IP)

2-10 µg/mg lysate

-

Product Specifications

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Region between residue 2127 and the C-terminus (residue 2177) of human Mediator of RNA Polymerase II Transcription, subunit 12. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:MED12,uniProtId:Q93074-1,ncbiNodeId:9606,antigenRange:2127-2177,antigenLength:2177,antigenImageFileName:A300-774A-M_MED12_Q93074-1_Rabbit.svg,antigenImageFileNamePDP:A300-774A-M_MED12_Q93074-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Storage conditions

4° C

Shipping conditions

Wet ice

Product Specific Information

The recommended shelf life for this product is 1 year from date of receipt.

Application Note: For IHC, epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

Based on 100% sequence identity, this antibody is predicted to react with Chimpanzee

Target Information

The initiation of transcription is controlled in part by a large protein assembly known as the preinitiation complex. A component of this preinitiation complex is a 1.2 MDa protein aggregate called Mediator. This Mediator component binds with a CDK8 subcomplex which contains the protein encoded by this gene, mediator complex subunit 12 (MED12), along with MED13, CDK8 kinase, and cyclin C.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.