Thermo Fisher Scientific MED12 Polyclonal Antibody
Rabbit polyclonal antibody targeting human MED12, validated for WB, IHC(P), and IP. Recognizes residues 2127–2177 of MED12. Supplied as liquid, unconjugated form at 1 mg/mL. Recommended storage at 4°C and shelf life of 1 year.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000–1:10,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:1,000–1:5,000 |
| Immunoprecipitation (IP) | 2–10 µg/mg lysate |
Product Specifications
| Specification | Description |
|---|---|
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Region between residue 2127 and the C-terminus (residue 2177) of human Mediator of RNA Polymerase II Transcription, subunit 12 (MED12) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
- Recommended shelf life: 1 year from date of receipt
- For IHC: Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections
- Based on 100% sequence identity, this antibody is predicted to react with Chimpanzee
Target Information
The initiation of transcription is controlled in part by a large protein assembly known as the preinitiation complex. A component of this complex is a 1.2 MDa protein aggregate called Mediator. This Mediator component binds with a CDK8 subcomplex containing the protein encoded by the MED12 gene, along with MED13, CDK8 kinase, and cyclin C.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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