Thermo Fisher Scientific SMAD5 Polyclonal Antibody
SMAD5 단백질을 인식하는 Thermo Fisher Scientific의 rabbit polyclonal 항체로, WB, IHC, ICC/IF, ELISA에 사용 가능. Human, Mouse, Rat 반응성. Affinity 정제, PBS/glycerol buffer, -20°C 보관. 연구용 전용 제품.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
| ELISA | 1 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthetic peptide corresponding to a sequence within amino acids 200–300 of human Smad5 (NP_0058943) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.54 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.3, with 50% glycerol |
| Contains | 0.09% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2805666 |
Product Specific Information
Immunogen sequence:
STYPNSPASS GPGSPFQLPA DTPPPAYMPP DDQMGQDNSQ PMDTSNNMIP QIMPSISSRD VQPVAYEEPK HWCSIVYYEL NNRVGEAFHA SSTSVLVDGF T
Positive Samples: SKOV3, MCF-7, Mouse kidney
Cellular Location: Cytoplasm, Nucleus
Target Information
SMADs are members of the MAD-related family of molecules. These intracellular proteins are essential components in the signaling pathways of serine/threonine kinase receptors of the transforming growth factor beta (TGF-β) superfamily.
SMADs are classified as:
- Receptor-regulated SMADs (R-SMADs): SMAD1, 2, 5, 8, 9
- Common-mediator SMAD (Co-SMAD): SMAD4
- Inhibitory SMADs (I-SMADs): SMAD6, 7
SMAD1, 5, 8, and 9 share high sequence homology, and antibodies may cross-react among them. SMAD8 and SMAD9 are often used interchangeably in literature.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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