Thermo Fisher Scientific GADD45A Monoclonal Antibody (3D12)
GADD45A 단백질을 인식하는 Mouse Monoclonal Antibody로 Western Blot, ELISA, PLA 등 다양한 응용에 적합함. Human 반응성, Unconjugated 형태이며 PBS buffer에 보관. 스트레스 반응 및 세포주기 조절 연구에 활용 가능.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–5 µg/mL |
| ELISA | 1–5 µg/mL |
| in situ PLA (PLA) | 1:1200 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 3D12 |
| Immunogen | GADD45A (AAH11757, 76 a.a. approximately 165 a.a) partial recombinant protein with GST tag. MW of GST tag alone is 26 kDa. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | See Label |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.4 |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Sequence of this protein is as follows:
TLIQAFCCEN DINILRVSNP GRLAELLLLE TDAGPAASEG AEQPPDLHCV LVTNPHSSQW KDPALSQLIC FCRESRYMDQ WVPVINLPER
Target Information
GADD45 alpha, beta and gamma are an evolutionarily conserved family of nuclear proteins that function as stress sensors for cellular physiological and environmental damage.
GADD45 proteins are required for the activation of the G2/M checkpoint induced by UV radiation or alkylating agents.
GADD45-induced G2/M checkpoint is regulated through inactivation of Cdc2-cyclin B1 kinase.
GADD45 forms a complex with p21 and Cdc2, and may serve as a core for interaction with other cell cycle regulators.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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