Thermo Fisher Scientific GFP Polyclonal Antibody
GFP 단백질을 인식하는 Thermo Fisher Scientific의 Rabbit Polyclonal Antibody. 다양한 형식의 면역실험(WB, IHC, ICC, ELISA)에 적합하며 높은 특이성과 안정성을 제공. KLH 접합 합성 펩타이드 면역원 사용, Protein A 정제, -20°C 보관.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000–1:10,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200–1:400 |
| Immunohistochemistry (Frozen) (IHC (F)) | 1:100–1:500 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
| ELISA | 1:500–1:1,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Tag |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | KLH conjugated synthetic peptide derived from jellyfish Green Fluorescent Protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | 0.01M TBS, pH 7.4, with 50% glycerol, 1% BSA |
| Contains | 0.02% ProClin 300 |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
The jellyfish Aequorea victoria contains green fluorescent protein (GFP Tag) that emits light in the bioluminescence reaction of the animal.
GFP has been widely used as a reporter protein for gene expression in eukaryotic and prokaryotic organisms, and as a protein tag in cell culture and multicellular organisms.
As a fusion tag, GFP can be used to localize proteins, study their movement, and research the dynamics of subcellular compartments.
GFP technology has provided significant insights into the physiological activities of living cells.
- GFP is a 27 kDa monomeric protein that autocatalytically forms a fluorescent pigment.
- Absorbs blue light (max 395 nm) and emits green light (peak 508 nm).
- Fluorescence is stable, species-independent, and suitable for various applications.
- Extensively used for monitoring gene expression and protein localization.
- Also applied in protein-protein interaction studies (yeast two-hybrid system) and fluorescence energy transfer (FRET) experiments.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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