Thermo Fisher Scientific Phospho-BAD (Ser128) Polyclonal Antibody
BAD 단백질의 Ser128 인산화 부위를 특이적으로 인식하는 폴리클로날 항체. Western blot, IHC, ICC, Flow cytometry 및 ELISA에 사용 가능. Human, Mouse, Rat 반응성. Protein A 정제, 1 mg/mL 농도, -20°C 보관.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:200 |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:200 |
| Immunohistochemistry (Frozen) (IHC-F) | 1:100–1:500 |
| Immunocytochemistry (ICC/IF) | 1:100–1:500 |
| Flow Cytometry (Flow) | 1:20–1:100 |
| ELISA | 1:500–1:1,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | KLH-conjugated synthetic phosphopeptide derived from mouse BAD around the phosphorylation site of Ser128 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | 0.01M TBS, pH 7.4, with 50% glycerol, 1% BSA |
| Contains | 0.02% ProClin 300 |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
The BAD protein is a member of the BCL-2 family, known as regulators of programmed cell death. It promotes apoptosis by forming heterodimers with BCL-xL and BCL-2, counteracting their anti-apoptotic functions. The proapoptotic activity of BAD is regulated by phosphorylation, involving kinases such as AKT and MAP kinase, as well as phosphatase calcineurin. Alternative splicing generates two transcript variants encoding the same isoform.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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