Thermo Fisher Scientific SIRT1 Polyclonal Antibody
SIRT1 단백질을 인식하는 Rabbit Polyclonal Antibody로 WB, ICC/IF, IP에 적합. Human 및 Non-human primate 반응성. 항원 친화 크로마토그래피로 정제되었으며, 보존제 없이 -20°C에서 보관. Sirtuin 관련 연구용으로 최적화된 제품.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:1,000 | View 1 publication |
| Immunocytochemistry (ICC/IF) | 1:100 | - |
| Immunoprecipitation (IP) | 1:50 | - |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Non-human primate |
| Published Species | Not Applicable |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide corresponding to the carboxy terminus of human SirT1 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 21 µg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | 0.01M HEPES (pH 7.5), 0.15M NaCl, 100 µg/mL BSA, 50% glycerol |
| Contains | No preservative |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_10985143 |
Product Specific Information
- It is not recommended to aliquot this antibody.
- This antibody is not cross-reactive with other sirtuin proteins.
Target Information
NAD-dependent protein deacetylase sirtuin-1 (SIRT1) links transcriptional regulation directly to intracellular energetics and participates in the coordination of several cellular functions such as cell cycle, response to DNA damage, metabolism, apoptosis, and autophagy.
SIRT1 modulates chromatin function through deacetylation of histones and can promote alterations in methylation of histone and DNA, leading to transcriptional repression.
It is essential in skeletal muscle cell differentiation and mediates the inhibitory effect on skeletal myoblast differentiation under low nutrient conditions, involving AMPK and NAMPT pathways.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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