Thermo Fisher Scientific Firefly luciferase Monoclonal Antibody (CS 17)
Firefly luciferase 단백질을 특이적으로 인식하는 Mouse monoclonal antibody. Western blot, IHC, ICC/IF 등 다양한 응용에 적합. 비특이적 반응이 없으며 높은 특이성과 재현성을 제공. 단백질 발현 연구 및 리포터 분석에 활용 가능.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1–3 µg/mL | - |
| Immunohistochemistry (IHC) | - | 1 publication |
| Immunohistochemistry (Frozen) (IHC (F)) | 15–50 µg/mL | - |
| Immunocytochemistry (ICC/IF) | - | 6 publications |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Luciferase |
| Published Species | Human, Luciferase, Non-human primate, Rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | CS 17 |
| Immunogen | Luciferase protein from Photinus pyralis |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS, pH 7.4 |
| Contains | 0.1% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2533218 |
Product Specific Information
Reactivity was confirmed via Western Blot analysis on samples containing added purified luciferase protein (mouse heart, liver, kidney, and cortex tissues).
No cross-reactivity was observed with mouse heart, NIH3T3, Jurkat, and human fibroblasts.
In IHC (frozen tissue) and immunofluorescence applications, this antibody detects tissue-specific expression of Luciferase protein in Drosophila embryos using the Gal4-UAS system with two different Gal4 drivers.
Target Information
Luciferase produces green light with a wavelength of 562 nm and catalyzes the reaction:
D-firefly luciferin + O₂ + ATP → firefly oxyluciferin + CO₂ + AMP + diphosphate + light
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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