Thermo Fisher Scientific Bovine Serum Albumin Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

View 10 publications 10 publications

Immunohistochemistry (Paraffin) (IHC (P))

Assay-dependent

-

Immunocytochemistry (ICC/IF)

Assay-dependent

View 3 publications 3 publications

Flow Cytometry (Flow)

-

View 1 publication 1 publication

ELISA (ELISA)

Assay-dependent

View 1 publication 1 publication

Immunoprecipitation (IP)

Assay-dependent

View 1 publication 1 publication

Functional Assay (Functional)

-

View 1 publication 1 publication

Miscellaneous PubMed (Misc)

-

View 2 publications 2 publications

Product Specifications

Species Reactivity

Bovine

Published species

Baboon, Bovine, Human, Mouse, Non-human primate, Rabbit

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

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View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

2 mg/mL

Purification

IgG fraction

Storage buffer

PBS, pH 7.2

Contains

5mM sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2534139

Product Specific Information

Anti-bovine serum albumin (anti-BSA) antibody is a highly purified rabbit IgG fraction that can be used for the detection of BSA in tracer studies or in protease assays. This antibody demonstrates high specificity for BSA and little or no crossreactivity to other serum proteins. Cat. No. A11133 can be used in enzyme and fluorescence immunoassays and blots, cell or tissue immunostaining, and as a precipitating antibody or as an immuno-adsorbant when attached to solid supports. For detection of this rabbit antibody, we offer a variety of second-step reagents labeled with biotin or with a wide range of fluorophores.

Because staining protocols vary with application, the appropriate dilution of antibody should be determined empirically. We suggest using serial dilutions from about 1:100 to 1:1,000 to determine the optimal conditions for your experiments.

Target Information

Albumin is a soluble, monomeric protein which comprises about one-half of the blood serum protein. Albumin functions primarily as a carrier protein for steroids, fatty acids, and thyroid hormones and plays a role in stabilizing extracellular fluid volume. Albumin is a globular unglycosylated serum protein of molecular weight 65,000. Albumin is synthesized in the liver as preproalbumin which has an N-terminal peptide that is removed before the nascent protein is released from the rough endoplasmic reticulum. The product, proalbumin, is in turn cleaved in the Golgi vesicles to produce the secreted albumin.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.