Thermo Fisher Scientific GFP Monoclonal Antibody (3E6)

Applications

Tested Dilution

Publications

Western Blot (WB)

2 µg/mL

View 57 publications 57 publications

Immunohistochemistry (IHC)

-

View 240 publications 240 publications

Immunohistochemistry (Paraffin) (IHC (P))

-

View 2 publications 2 publications

Immunohistochemistry (PFA fixed) (IHC (PFA))

-

View 2 publications 2 publications

Immunohistochemistry (Frozen) (IHC (F))

-

View 12 publications 12 publications

Immunohistochemistry - Free Floating (IHC (Free))

-

View 9 publications 9 publications

Immunocytochemistry (ICC/IF)

1:50

View 129 publications 129 publications

Flow Cytometry (Flow)

-

View 3 publications 3 publications

ELISA (ELISA)

1:200-1:2,000

-

Immunoprecipitation (IP)

1:200-1:2,000

View 44 publications 44 publications

ChIP assay (ChIP)

-

View 6 publications 6 publications

in situ PLA (PLA)

-

View 3 publications 3 publications

Affinity Purification (AP)

-

View 1 publication 1 publication

In vitro Assay (IV)

-

View 1 publication 1 publication

Miscellaneous PubMed (Misc)

-

View 28 publications 28 publications

Product Specifications

Species Reactivity

Tag

Published species

Tag

Host/Isotype

Mouse / IgG2a

Class

Monoclonal

Type

Antibody

Clone

3E6

Immunogen

The GFP was isolated directly from the jellyfish Aequorea victoria.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Purification

Protein G

Storage buffer

PBS with BSA

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_221568

Product Specific Information

Storage and reconstitution: to prepare a 0.2 mg/mL stock solution, reconstitute the lyophilized antibodies in 0.5 mL PBS, pH 7.4. Store solution for up to 3 months at 4ºC with the addition of 2mM sodium azide.

Target Information

The jellyfish Aequorea victoria contains green fluorescent protein (GFP Tag) that emits light in the bioluminescence reaction of the animal. GFP has been used widely as a reporter protein for gene expression in eukaryotic and prokaryotic organisms, and as a protein tag in cell culture and in multicellular organisms. As a fusion tag, GFP can be used to localize proteins, to study their movement or to research the dynamics of the subcellular compartments where these proteins are targeted. GFP technology has revealed considerable new insights in the physiological activities of living cells. GFP is a 27 kDa monomeric protein, which autocatalytically forms a fluorescent pigment. The wild type protein absorbs blue light (maximally at 395nm) and emits green light (peak emission 508nm) in the absence of additional proteins, substrates, or co-factors. GFP fluorescence is stable, species independent and is suitable for a variety of applications. GFP has been used extensively as a fluorescent tag to monitor gene expressin and protein localization. Moreover, other applications for GFP include its use in assessing protein protein interactions in the yeast two hybrid system, and in measuring distances between proteins in fluorescence energy transfer (FRET) experiments.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.