Thermo Fisher Scientific GFP Monoclonal Antibody (3E6)
GFP 단백질을 인식하는 Mouse 모노클로날 항체로, 다양한 형광 단백질 표지 연구에 활용 가능. Western blot, ICC/IF, IP 등 여러 응용 분야에서 검증됨. 동결건조 형태로 제공되며, 재구성 후 4℃에서 안정적으로 보관 가능. 연구용 전용 시약.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 2 µg/mL | 57 |
| Immunohistochemistry (IHC) | - | 240 |
| Immunohistochemistry (Paraffin) (IHC (P)) | - | 2 |
| Immunohistochemistry (PFA fixed) (IHC (PFA)) | - | 2 |
| Immunohistochemistry (Frozen) (IHC (F)) | - | 12 |
| Immunohistochemistry - Free Floating (IHC (Free)) | - | 9 |
| Immunocytochemistry (ICC/IF) | 1:50 | 129 |
| Flow Cytometry (Flow) | - | 3 |
| ELISA | 1:200–1:2,000 | - |
| Immunoprecipitation (IP) | 1:200–1:2,000 | 44 |
| ChIP assay (ChIP) | - | 6 |
| in situ PLA (PLA) | - | 3 |
| Affinity Purification (AP) | - | 1 |
| In vitro Assay (IV) | - | 1 |
| Miscellaneous PubMed (Misc) | - | 28 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Tag |
| Published Species | Tag |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 3E6 |
| Immunogen | GFP isolated from Aequorea victoria |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Purification | Protein G |
| Storage Buffer | PBS with BSA |
| Contains | No preservative |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_221568 |
Product Specific Information
To prepare a 0.2 mg/mL stock solution, reconstitute the lyophilized antibody in 0.5 mL PBS (pH 7.4). Store the reconstituted solution at 4°C for up to 3 months with the addition of 2 mM sodium azide.
Target Information
The jellyfish Aequorea victoria expresses Green Fluorescent Protein (GFP), which emits green light (peak 508 nm) upon excitation with blue light (395 nm). GFP is widely used as a reporter protein for gene expression and as a protein tag in both prokaryotic and eukaryotic systems. It enables visualization of protein localization, movement, and subcellular dynamics. GFP is a 27 kDa monomeric protein that forms a fluorescent chromophore without requiring additional cofactors. Its fluorescence is stable and species-independent, making it suitable for applications such as FRET, yeast two-hybrid assays, and live-cell imaging.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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