Thermo Fisher Scientific CD223 (LAG-3) Monoclonal Antibody (3DS223H), Brilliant Violet 786, eBioscience

Thermo Fisher Scientific CD223 (LAG-3) Monoclonal Antibody (3DS223H), Brilliant Violet 786, eBioscience

Applications

• Flow Cytometry (Flow)

Tested Dilution: 5 µL (0.25 µg)/test

Product Specifications

Additional Formats Available

• Alexa Fluor 647, 660, 700
• APC, APC-eFluor 780
• Brilliant UV 395, 563, 661, 737, 805
• Brilliant Violet 421, 480, 605, 650, 711
• eFluor 450, 506
• FITC
• NovaFluor Blue, Red, Yellow series
• PE, PE-Cyanine5, PE-Cyanine7, PE-eFluor 610
• PerCP-eFluor 710
• Super Bright 436, 600, 645, 702, 780
• Request custom conjugation available

Product Specific Information

This 3DS223H monoclonal antibody recognizes human CD223, also known as Lymphocyte Activation Gene 3 (LAG-3). LAG-3 is a 70-kDa surface glycoprotein of the Ig superfamily with homology to CD4. It binds to MHC class II with higher affinity than CD4 and negatively regulates T cell activation and homeostatic proliferation.

Surface expression of LAG-3 is reported on activated T cells (including regulatory T cells) and NK cells. CD8+ T cells typically express LAG-3 at higher levels than CD4+ T cells. Coexpression of LAG-3 and CD49b identifies human and mouse Type 1 regulatory T cells (Tr1 cells).

This antibody recognizes a formaldehyde-fixed epitope.

Applications Reported: Flow cytometric analysis
Applications Tested: Pre-diluted and tested on stimulated human peripheral blood cells at 5 µL (0.25 µg) per test.

Brilliant Violet™ 786 (BV786) emits at 786 nm, optimized for instruments equipped with a violet (405 nm) laser. Ensure compatibility with your cytometer.

Buffer Recommendations:
Use Super Bright Complete Staining Buffer (Product # SB-4401-42) or Brilliant Stain Buffer™ (Product # 00-4409-75) to minimize non-specific polymer interactions.

Light Sensitivity: Protect from light to prevent photo-induced oxidation.
Fixation: Compatible with IC Fixation Buffer (Product # 00-8222-49) or 1-step Fix/Lyse Solution (Product # 00-5333-54). Not compatible with methanol-based fixation.

Excitation/Emission: 407 nm / 786 nm
Laser: Violet Laser

BRILLIANT VIOLET™ is a trademark of Becton, Dickinson and Company, used under license. Powered by Sirigen™.

Target Information

LAG-3 is a 70-kDa surface glycoprotein of the Ig superfamily homologous to CD4. It binds MHC class II with high affinity and regulates T cell activation negatively. Expressed on activated T cells and NK cells, with higher levels on CD8+ T cells. Coexpression with CD49b marks Type 1 regulatory T cells (Tr1).

For Research Use Only. Not for use in diagnostic procedures or resale without authorization.

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