Thermo Fisher Scientific CD223 (LAG-3) Monoclonal Antibody (3DS223H), Brilliant Violet 786, eBioscience
인간 CD223(LAG-3)을 인식하는 3DS223H 단클론 항체로, Brilliant Violet 786 형광체가 결합되어 있습니다. Flow Cytometry에 최적화되어 있으며, Violet(405 nm) 레이저 사용 시 높은 감도를 제공합니다. 형광 상호작용 최소화를 위해 Brilliant Stain Buffer 사용을 권장합니다.
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Thermo Fisher Scientific CD223 (LAG-3) Monoclonal Antibody (3DS223H), Brilliant Violet 786, eBioscience
Applications
- Flow Cytometry (Flow)
Tested Dilution: 5 µL (0.25 µg)/test
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host/Isotype | Mouse / IgG1, kappa |
| Recommended Isotype Control | Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), Brilliant Violet™ 786, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 3DS223H |
| Conjugate | Brilliant Violet™ 786 |
| Excitation/Emission Max | 406 / 788 nm |
| Form | Liquid |
| Concentration | 5 µL/Test |
| Purification | Affinity chromatography |
| Storage buffer | PBS, pH 7.2, with BSA |
| Contains | 0.09% sodium azide |
| Storage conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2925735 |
Additional Formats Available
- Alexa Fluor 647, 660, 700
- APC, APC-eFluor 780
- Brilliant UV 395, 563, 661, 737, 805
- Brilliant Violet 421, 480, 605, 650, 711
- eFluor 450, 506
- FITC
- NovaFluor Blue, Red, Yellow series
- PE, PE-Cyanine5, PE-Cyanine7, PE-eFluor 610
- PerCP-eFluor 710
- Super Bright 436, 600, 645, 702, 780
- Request custom conjugation available
Product Specific Information
This 3DS223H monoclonal antibody recognizes human CD223, also known as Lymphocyte Activation Gene 3 (LAG-3). LAG-3 is a 70-kDa surface glycoprotein of the Ig superfamily with homology to CD4. It binds to MHC class II with higher affinity than CD4 and negatively regulates T cell activation and homeostatic proliferation.
Surface expression of LAG-3 is reported on activated T cells (including regulatory T cells) and NK cells. CD8+ T cells typically express LAG-3 at higher levels than CD4+ T cells. Coexpression of LAG-3 and CD49b identifies human and mouse Type 1 regulatory T cells (Tr1 cells).
This antibody recognizes a formaldehyde-fixed epitope.
Applications Reported: Flow cytometric analysis
Applications Tested: Pre-diluted and tested on stimulated human peripheral blood cells at 5 µL (0.25 µg) per test.
Brilliant Violet™ 786 (BV786) emits at 786 nm, optimized for instruments equipped with a violet (405 nm) laser. Ensure compatibility with your cytometer.
Buffer Recommendations:
Use Super Bright Complete Staining Buffer (Product # SB-4401-42) or Brilliant Stain Buffer™ (Product # 00-4409-75) to minimize non-specific polymer interactions.
Light Sensitivity: Protect from light to prevent photo-induced oxidation.
Fixation: Compatible with IC Fixation Buffer (Product # 00-8222-49) or 1-step Fix/Lyse Solution (Product # 00-5333-54). Not compatible with methanol-based fixation.
Excitation/Emission: 407 nm / 786 nm
Laser: Violet Laser
BRILLIANT VIOLET™ is a trademark of Becton, Dickinson and Company, used under license. Powered by Sirigen™.
Target Information
LAG-3 is a 70-kDa surface glycoprotein of the Ig superfamily homologous to CD4. It binds MHC class II with high affinity and regulates T cell activation negatively. Expressed on activated T cells and NK cells, with higher levels on CD8+ T cells. Coexpression with CD49b marks Type 1 regulatory T cells (Tr1).
For Research Use Only. Not for use in diagnostic procedures or resale without authorization.
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