Thermo Fisher Scientific TNF alpha Canine ProcartaPlex Simplex Kit

Thermo Fisher Scientific TNF alpha Canine ProcartaPlex Simplex Kit

Invitrogen™ ProcartaPlex™ Simplex Kit

The Canine TNF alpha Simplex ProcartaPlex Kit measures TNF alpha protein and is designed to be combinable with other Simplex kits to create custom multiplex panels utilizing Luminex xMAP technology for protein detection and quantitation. When combining multiple Simplex kits, only one buffer kit (sold separately) is needed per assay plate regardless of plex size.

About ProcartaPlex Assays for the Luminex Platform

ProcartaPlex immunoassays are based on sandwich ELISA principles, using two highly specific antibodies binding to different epitopes of one protein to quantitate multiple targets simultaneously on a Luminex instrument.

• More results per sample — measure multiple protein targets simultaneously in a single 25–50 μL sample
• Well-established Luminex technology — highly referenced multiplexing platform for protein detection and quantitation

ProcartaPlex assays utilize Luminex xMAP (multianalyte profiling) technology for simultaneous detection and quantitation of up to 80 protein targets in a single 25–50 μL sample from plasma, serum, cell culture supernatants, and other bodily fluids.

The Luminex beads in the ProcartaPlex assay are internally dyed with precise proportions of red and infrared fluorophores to create spectrally unique signatures identifiable by Luminex xMAP detection systems (e.g., Luminex 200, FLEXMAP 3D, MAGPIX).
Similar to a sandwich ELISA, the ProcartaPlex assay uses matched antibody pairs to identify the protein of interest. Each spectrally unique bead is labeled with antibodies specific for a single target protein, and bound proteins are detected with biotinylated antibodies and streptavidin–R-phycoerythrin (RPE).

The key difference between a ProcartaPlex assay and a traditional ELISA is that the capture antibody is conjugated to a bead rather than adsorbed to a microplate well, allowing reagents to be free-floating in solution. The Luminex instrument uses two lasers: one to distinguish bead signatures and another to quantify RPE fluorescence proportional to protein concentration. This enables profiling of more target proteins using less sample and time compared to ELISA.

ProcartaPlex multiplex panels are available across six species (human, mouse, rat, nonhuman primate, porcine, and canine).
For more information, visit thermofisher.com/procartaplex.

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