Thermo Fisher Scientific Histone H2A Polyclonal Antibody
Western blot과 IHC(P)에서 높은 특이성과 재현성을 보이는 Histone H2A 폴리클로날 항체. Human, Mouse, Rat에 반응하며, 항원 친화 크로마토그래피로 정제됨. 1 mg/mL 농도의 액상 형태로, 4°C 또는 -20°C에서 보관 가능.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:5,000–1:20,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:1,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Full length human Histone H2A recombinant protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7, with 20% glycerol |
| Contains | 0.025% ProClin 300 |
| Storage Conditions | Store at 4°C (short term). For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| RRID | AB_2553203 |
Product Specific Information
- Recommended positive controls: MCF-7, MDA-MB-231
- Predicted reactivity: Mouse (100%), Rhesus Monkey (100%)
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Histones are basic nuclear proteins responsible for the nucleosome structure of chromosomal fibers in eukaryotes. Two molecules each of H2A, H2B, H3, and H4 form an octamer around which approximately 146 bp of DNA is wrapped in repeating units called nucleosomes. The linker histone H1 interacts with linker DNA between nucleosomes and contributes to chromatin compaction.
This gene is intronless and encodes a member of the histone H4 family. Transcripts lack polyA tails but contain a palindromic termination element. The gene is located in the large histone gene cluster on chromosome 6.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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