Thermo Fisher Scientific GBL Polyclonal Antibody
Thermo Fisher Scientific의 GBL Polyclonal Antibody는 인간, 마우스, 랫트 시료에 반응하는 Rabbit IgG 항체입니다. Western blot, IHC, ICC에 적합하며, mTOR 신호전달 관련 연구에 활용됩니다. 고순도 친화 크로마토그래피 정제 및 안정적 보관이 가능합니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:800 |
| Immunocytochemistry (ICC/IF) | 1:200 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein within human MLST8 aa 1–250/326 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.4, with 0.1% BSA, 40% glycerol |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long-term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_3094046 |
Product Specific Information
- Positive control: C2C12 cell lysate, L6 cell lysate, mouse brain tissue lysate, human brain tissue lysate, rat brain tissue lysate, 293 cell lysate, MCF-7 cell lysate, PC-3M, rat heart tissue, mouse skeletal muscle tissue
- Predicted band size: 36 kDa
- Subcellular Location: Cytoplasm
Target Information
G-protein beta subunit-like (GBL protein) is a member of the WD-40 protein family. It is a highly conserved cytosolic protein composed of seven WD-40 repeating motifs. LST8 functions as a component of the mTOR signaling complex (TORC1) and positively regulates the mTOR pathway. It interacts with the kinase domain of mTOR and stabilizes its interaction with raptor. LST8 is involved in regulating cell growth and in nutrient and growth factor-mediated mTOR signaling to S6K1. Reports indicate that insulin upregulates LST8 expression, suggesting its role in intracellular events related to insulin-receptor activation, including insulin-stimulated glucose transport and glycogen synthesis. Northern blot analysis shows ubiquitous expression of LST8, with the highest levels in brain and testis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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