Thermo Fisher Scientific GBL Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

-

Immunohistochemistry (Paraffin) (IHC (P))

1:800

-

Immunocytochemistry (ICC/IF)

1:200

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein within human MLST8 aa 1-250/326. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:GBL,uniProtId:Q9BVC4-1,ncbiNodeId:9606,antigenRange:1-250,antigenLength:326,antigenImageFileName:PA5-144651_GBL_Q9BVC4-1_Rabbit.svg,antigenImageFileNamePDP:PA5-144651_GBL_Q9BVC4-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.4, with 0.1% BSA, 40% glycerol

Contains

0.05% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_3094046

Product Specific Information

Positive control: C2C12 cell lysate, L6 cell lysate, mouse brain tissue lysate, human brain tissue lysate, rat brain tissue lysate, 293 cell lysate, MCF-7 cell lysate, PC-3M, rat heart tissue, mouse skeletal muscle tissue.

Predicted band size: 36 kDa

Subcellular Location: Cytoplasm.

Target Information

G-protein beta subunit like (GBL protein) is a novel member of WD-40 protein family. It is a highly conserved cytosolic protein consisting of seven WD-40 repeating motifs. LST8 is a functional component of mTOR signaling complex (TORC1) and positively up-regulates the mTOR pathway. It interacts with the kinase domain of mTOR and stabilizes its interaction with raptor. LST8 participates in regulating cell growth and also in nutrient and growth factor-mediated mTOR signaling to S6K1. Reports suggest up-regulated expression of LST8 due to insulin indicating a possible role of LST8 in intracellular events related to insulin-receptor activation including insulin-stimulated glucose transport and glycogen synthesis. Northern Blot analysis detected a ubiquitous expression of LST8 with highest expression in brain and testis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.