Thermo Fisher Scientific Phospho-PLK1 (Ser482, Ser486, Ser490) Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

-

Product Specifications

Species Reactivity

Rat, Xenopus

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser482/486/490 of Xenopus PLK1 conjugated to KLH if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:PLK1,uniProtId:P70032-1,ncbiNodeId:8355,antigenRange:482,antigenLength:598,antigenImageFileName:PA1-4637_PLK1_P70032-1_Rabbit.svg,antigenImageFileNamePDP:PA1-4637_PLK1_P70032-1_Rabbit_PDP.jpeg,sortOrder:2}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Purification

Affinity chromatography

Storage buffer

0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol

Contains

no preservative

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_560673

Product Specific Information

This antibody is predicted to react with rat, mouse, human and zebrafish based on 100% sequence homology.

This antibody is specific for the ~120 kDa PLKK protein phosphorylated at Ser482,486,490 in Western blots of Xenopus lysates.

Target Information

PLK1 is a serine/threonine kinase that is thought to function in cell mitosis and regarded as a marker of cell proliferation. It has been found to play a critical role in carcinogenesis. Polo-like kinase proteins acts by binding and phosphorylating proteins are that already phosphorylated on a specific motif recognized by the POLO box domains. Phosphorylates BORA, BUB1B/BUBR1, CCNB1, CDC25C, CEP55, ECT2, ERCC6L, FBXO5/EMI1, FOXM1, KIF20A/MKLP2, CENPU, NEDD1, NINL, NPM1, NUDC, PKMYT1/MYT1, KIZ, PPP1R12A/MYPT1, PRC1, RACGAP1/CYK4, SGOL1, STAG2/SA2, TEX14, TOPORS, p73/TP73, TPT1 and WEE1. Plays a key role in centrosome functions and the assembly of bipolar spindles by phosphorylating KIZ, NEDD1 and NINL.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.