Thermo Fisher Scientific N-Cadherin Recombinant Rabbit Monoclonal Antibody (BLR097G)

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-dependent

-

Immunocytochemistry (ICC/IF)

1:100 - 1:500

-

Flow Cytometry (Flow)

1 µL per 1 x 10^6 cells

-

Immunoprecipitation (IP)

12 µL/1 mg lysate

-

Product Specifications

Species Reactivity

Human, Mouse

Host/Isotype

Rabbit / IgG

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

BLR097G

Immunogen

Recombinant protein representing the extracellular domain of human N-Cadherin (Cadherin-2, residues 160-724). if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:N-cadherin,uniProtId:P19022-1,ncbiNodeId:9606,antigenRange:160-724,antigenLength:906,antigenImageFileName:A700-097_N-cadherin_P19022-1_Rabbit.svg,antigenImageFileNamePDP:A700-097_N-cadherin_P19022-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

500 µg/mL

Purification

purified

Storage buffer

BBS, pH 7.8 to 8.6, with 0.1% BSA

Contains

0.09% sodium azide

Storage conditions

4° C

Shipping conditions

Wet ice

Target Information

N-cadherin is a 140 kDa protein belonging to a family of transmembrane molecules that mediate calcium-dependent intercellular adhesion. Cadherins are involved in controlling morphogenetic movements during development and regulate cell surface adhesion through homotypic adhesion with the same cadherin species. N-cadherin`s function is dependent on its association with the actin-cytoskeleton and is mediated through interactions between the C-terminal region of N-cadherin and the cytoplasmic catenin proteins. The stability of this association is regulated by phosphorylation and dephosphorylation of beta-catenin. Further, N-cadherin is a calcium dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. The protein functions during gastrulation and is required for establishment of left-right asymmetry.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.