Thermo Fisher Scientific GPR77 Monoclonal Antibody (6C1G10)
인간 C5AR2 단백질을 인식하는 Thermo Fisher의 GPR77 단클론 항체입니다. Western blot, IHC, ICC/IF, Flow cytometry, ELISA 등 다양한 응용에 적합합니다. 고순도 Protein G 정제, 액상 형태로 제공되며 연구용으로만 사용 가능합니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200–1:1,000 |
| Immunocytochemistry (ICC/IF) | Assay-dependent |
| Flow Cytometry (Flow) | 1:200–1:400 |
| ELISA | 1:10,000 |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 6C1G10 |
| Immunogen | Purified recombinant fragment of human C5AR2 (AA: extra mix) expressed in E. coli |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_3091229 |
Target Information
C5AR2 is an orphan member of the anaphylatoxin receptor family. It is a seven-transmembrane G-protein-coupled receptor with an N-terminal N-glycosylation site, several conserved cysteines, and multiple potential C-terminal phosphorylation sites.
C5AR2 binds to C5a and C5a-des-Arg and acts as a highly regulated scavenger receptor, negatively modulating C5aR-mediated inflammation.
Northern blot analysis indicates co-expression with C5aR in various tissues and cells, including neutrophils/macrophages, mast cells, dendritic cells, brain, lung, heart, kidney, liver, ovary, and testis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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