Thermo Fisher Scientific Goat anti-Rat IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 633
Alexa Fluor 633으로 결합된 염소 유래 항-쥐 IgG(H+L) 2차 항체로, WB, IHC, ICC/IF에 사용 가능. 높은 특이성과 신호 증폭 효과 제공. 교차흡착 처리로 비특이적 결합 최소화. 연구용으로만 사용.
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Applications 및 권장 희석 배수
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:5,000–1:10,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1–10 µg/mL |
| Immunocytochemistry (ICC/IF) | 1–2 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Rat |
| Host / Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Secondary Antibody |
| Immunogen | Gamma Immunoglobins Heavy and Light chains |
| Conjugate | Alexa Fluor™ 633 |
| Excitation/Emission Max | 631/650 nm |
| Form | Liquid |
| Concentration | 2 mg/mL |
| Purification | Purified |
| Storage Buffer | PBS, pH 7.5 |
| Contains | 5 mM sodium azide |
| Storage Conditions | 4°C, store in dark |
| Shipping Conditions | Ambient |
| RRID | AB_2535749 |
| Target | IgG |
| Cross Adsorption | Against mouse IgG, mouse serum, and human serum prior to conjugation |
| Antibody Form | Whole Antibody |
Product Specific Information
Product will be shipped at room temperature.
Target Information
Anti-Rat secondary antibodies are affinity-purified antibodies with well-characterized specificity for rat immunoglobulins and are useful in the detection, sorting, or purification of their specified target.
Secondary antibodies offer increased versatility, enabling users to employ many detection systems (e.g., HRP, AP, fluorescence).
They also provide greater sensitivity through signal amplification, as multiple secondary antibodies can bind to a single primary antibody.
Most commonly, secondary antibodies are generated by immunizing the host animal with pooled immunoglobulins from the target species and can be further purified and modified (e.g., immunoaffinity chromatography, antibody fragmentation, label conjugation) to generate highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Additional Available Formats
- Alexa Fluor 350
- Alexa Fluor 488
- Alexa Fluor 546
- Alexa Fluor 555
- Alexa Fluor 568
- Alexa Fluor 594
- Alexa Fluor 647
- Alexa Fluor 680
- AP
- APC
- Cyanine3
- Cyanine5
- DSB-X Biotin
- DyLight 488
- DyLight 550
- DyLight 594
- DyLight 650
- DyLight 680
- DyLight 755
- DyLight 800
- FITC
- HRP
- PE
- Texas Red-X
- TRITC
- Custom conjugation available upon request.
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