Thermo Fisher Scientific ALKBH5 Recombinant Rabbit Monoclonal Antibody (10H24L9)
ALKBH5 단백질을 인식하는 재조합 토끼 단클론 항체로, Western blot에 적합합니다. 인간 및 마우스 반응성이 검증되었으며, 높은 특이성과 재현성을 제공합니다. 단백질 A 정제, 액상 형태로 제공되며, 연구용으로만 사용 가능합니다.
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Applications
Western Blot (WB)
- Tested Dilution: 1:5,000
- View 2 publications
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Published Species | Mouse |
| Host / Isotype | Rabbit / IgG |
| Expression System | Expi293 |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | 10H24L9 |
| Immunogen | Protein corresponding to human ALKBH5 [aa66–aa292] |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS, pH 7.4 |
| Contains | 0.09% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2762417 |
Product Specific Information
This antibody is predicted to react with Monkey, Cat, and Pig.
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The antibody DNA sequences from immunoreactive rabbits are cloned and screened to select the best candidates for production.
Advantages include:
- Improved specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulation
- Broader immunoreactivity due to the larger rabbit immune repertoire
Target Information
ALKBH5 (RNA demethylase ALKBH5) is a dioxygenase that demethylates RNA by oxidative demethylation.
It specifically removes N(6)-methyladenosine (m6A) from RNA, the most prevalent internal modification in eukaryotic mRNA.
Demethylation of m6A affects mRNA processing and export. ALKBH5 can also demethylate N(6)-methyladenosine in single-stranded DNA (in vitro).
It requires molecular oxygen, alpha-ketoglutarate, and iron for activity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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