Thermo Fisher Scientific ATG4B Monoclonal Antibody (231CT21.1.7)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:100-1:250

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgM

Class

Monoclonal

Type

Antibody

Clone

231CT21.1.7

Immunogen

ATG4B recombinant protein

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Purification

Euglobulin precipitation

Storage buffer

PBS, pH 7.4

Contains

0.09% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2897442

Target Information

Cysteine protease ATG4b is an enzyme which is coded by ATG4b gene. Autophagy is a critical cellular homeostatic process that controls the turnover of damaged organelles and protein. ATG4b is a deubiquitin-like protease that plays dual roles in the core machinery of autophagy. ATG4b, cleaves the C-terminal amino acid of ATG8 family proteins MAP1LC3, GABARAPL1, GABARAPL2 and GABARAP, to reveal a C-terminal glycine. Exposure of the glycine at the C-terminus is essential for ATG8 proteins conjugation to phosphatidylethanolamine (PE) and insertion to membranes, which is necessary for autophagy. ATG4b is mainly expressed in the skeletal muscle, brain, heart, liver and pancreas.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.