Thermo Fisher Scientific CD3E Polyclonal Antibody

Applications and Tested Dilutions

Product Specifications

Product Specific Information

• Recommended shelf life: 1 year from date of receipt
• Application Note: For IHC, epitope retrieval with citrate buffer (pH 6.0) is recommended for FFPE tissue sections.

Target Information

The CD3 subunit complex is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and regulating the cell surface expression of the TCR complex.
T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits — CD3 gamma, CD3 delta, CD3 epsilon, and CD3 zeta.
These subunits are structurally related members of the immunoglobulin superfamily encoded by closely linked genes on human chromosome 11.

The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules, mediated by a double tyrosine-based motif present in each subunit.
CD3 may play roles in TCR-induced growth arrest, cell survival, and proliferation.

Expression profile:

• Present on 68–82% of normal peripheral blood lymphocytes
• 65–85% of thymocytes
• Expressed in Purkinje cells of the cerebellum
• Not expressed on B or NK cells

Decreased percentages of T lymphocytes may be observed in some autoimmune diseases.
Genes encoding CD3 epsilon, gamma, and delta are located on chromosome 11.
Defects in the CD3 gene are associated with CD3 immunodeficiency.

For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.