Thermo Fisher Scientific EnzChek Ultra Amylase Assay Kit

Invitrogen™ EnzChek™ Ultra Amylase Assay Kit

The EnzChek™ Ultra Amylase Assay Kit provides a solution-based assay featuring the speed, high sensitivity, and convenience required for measuring amylase activity or for screening amylase inhibitors in a high-throughput format.

This kit contains a starch derivative—the DQ™ starch substrate—that is labeled with BODIPY™ FL dye to such a degree that the fluorescence is quenched. This substrate is efficiently degraded by amylase; digestion relieves the quenching and yields highly fluorescent fragments. The accompanying increase in fluorescence is proportional to amylase activity and can be monitored with a fluorescence microplate reader or fluorometer using standard fluorescein filters.

Kit Specifications

• Label (Ex/Em): BODIPY™ FL conjugate (∼502/512 nm)
• Contents: Lyophilized substrate, 10X reaction buffer, substrate solvent, fluorescent standard, and detailed protocol
• Assay capacity: Sufficient reagents for 500 assays (100 μL assay volume, 96-well format)

Related Products

Find fluorescent substrates for other glycosidases such as xylanase, lysozyme, and β-galactosidase.
For more information, refer to Detecting Glycosidases—Section 10.2 in the Molecular Probes™ Handbook.

For Research Use Only. Not for human or animal therapeutic or diagnostic use.

제품 사양