Invitrogen™

EnzChek™ Ultra Amylase Assay Kit

The EnzChek™ Ultra Amylase Assay Kit provides a solution-based assay featuring the speed, high sensitivity, and convenience required for measuring자세히 알아보기

The EnzChek™ Ultra Amylase Assay Kit provides a solution-based assay featuring the speed, high sensitivity, and convenience required for measuring amylase activity or for screening amylase inhibitors in a high-throughput format. This EnzChek™ kit contains a starch derivative—the DQ™ starch substrate—that is labeled with BODIPY™ FL dye to such a degree that the fluorescence is quenched. This substrate is efficiently degraded by amylase; digestion relieves the quenching and yields highly fluorescent fragments. The accompanying increase in fluorescence is proportional to amylase activity and can be monitored with a fluorescence microplate reader or fluorometer, using standard fluorescein filters.

EnzChek™ Ultra Amylase Assay Kit Specifications:
• Label (Ex/Em): BODIPY™ FL conjugate (∼502/512 nm)
• Kit contains lyophilized substrate, 10X reaction buffer, substrate solvent, a fluorescent standard, and a detailed protocol
• Sufficient reagents are supplied for 500 assays (using a 100 μL assay volume in a 96-well microplate assay format)

Find Fluorescent Substrates for Other Glycosidases
In addition to the EnzChek™ Ultra Amylase Assay Kit, we offer kits and substrates to measure xylanase, lysozyme, β-galactosidase, and more. Review Detecting Glycosidases—Section 10.2 in the Molecular Probes™ Handbook for more information on these products.

For Research Use Only. Not for human or animal therapeutic or diagnostic use.

사양

검출 방법Fluorescence Intensity

용도(애플리케이션)Ultra Amylase Assay

용도 (장비)Fluorescence Microplate Reader

제품 유형Amylase Assay

제품라인EnzChek™

수량1 Kit

배송 조건Room Temperature

기질 특성Molecular Substrate

기질 유형Amylase Substrate

타겟 효소Amylase

Unit SizeEach