Thermo Fisher Scientific p14ARF Polyclonal Antibody
Human, Mouse, Rat에서 반응하는 p14ARF 폴리클로날 항체. Western blot, IHC, ICC/IF에 사용 가능. 항원 친화 크로마토그래피로 정제된 Rabbit IgG. 세포 내 위치는 세포질 및 핵. 연구용으로만 사용.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.1–1 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 2–10 µg/mL |
| Immunocytochemistry (ICC/IF) | 1:200 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthetic peptide corresponding to the internal segment of human p14ARF |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS with proprietary stabilizer |
| Contains | 0.01% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Positive Control: HeLa whole cell lysate transfected with CDKN2A/p14ARF with His-tag
- Cellular Location: Cytoplasmic and Nuclear
Target Information
The INK4a locus on chromosome 9p21 is frequently affected in human tumors. It encodes two structurally distinct tumor suppressor proteins, p16INK4a and the alternative reading frame protein, ARF (p14ARF in human and P19ARF in mouse). These two proteins interact with upstream tumor suppressors, retinoblastoma protein and p53, respectively. Both play roles in cell senescence, cell cycle control, and tumor suppression. The p14ARF (or P19ARF in mouse) proteins can induce G1 and G2 phase arrest dependent on functional p53. Functional differences between mouse and human ARF proteins may contribute to species-specific tumor susceptibility.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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