Thermo Fisher Scientific c-Myc Monoclonal Antibody (9E10.3)
Thermo Fisher Scientific의 c-Myc Monoclonal Antibody (Clone 9E10.3)는 인간 c-Myc 단백질의 C-말단을 인식하는 항체로, WB, IHC, IP 등 다양한 응용에 적합합니다. 단백질 G로 정제된 액상 형태이며, PBS 버퍼에 보존되어 연구용으로 사용됩니다.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:25–1:50 | View 11 publications |
| Immunohistochemistry (IHC) | – | View 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:25–1:50 | View 1 publication |
| Immunocytochemistry (ICC/IF) | – | View 3 publications |
| Flow Cytometry (Flow) | Assay-dependent | View publications |
| Immunoprecipitation (IP) | 10 µL | View 5 publications |
| ChIP assay (ChIP) | – | View 5 publications |
| Immunomicroscopy (IM) | Assay-dependent | View publications |
| Miscellaneous PubMed (Misc) | – | View 16 publications |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Human |
| Published Species | Human, Plant, Tag, Yeast |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 9E10.3 |
| Immunogen | A synthetic peptide corresponding to aa 408–439 (AEEQKLISEEDLLRKRREQLKHKLEQLRNSCA) from C-terminus of human c-Myc, coupled to KLH |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.3 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS, pH 7.4, with 0.2% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2536303 |
Additional Formats
Product Specific Information
This antibody detects an epitope at aa 410–419.
Staining of formalin-fixed paraffin-embedded tissue sections requires boiling the sections in 10 mM citrate buffer (pH 6.0) for 10–20 minutes, followed by cooling at room temperature for 20 minutes.
Recommended positive controls include Raji cells and human breast carcinoma.
Target Information
The c-Myc protein is a 62 kDa transcription factor encoded by the c-Myc gene on human chromosome 8q24. It is frequently activated in tumor cells and plays an essential role in cellular proliferation, differentiation, apoptosis, and cell cycle progression.
Key biological insights:
- Phosphorylation at Thr58/Ser62 is associated with regulation by GSK3, CDK, ERK2, and JNK.
- c-Myc is critical for vasculogenesis and angiogenesis in tumor development.
- Deregulated c-Myc expression induces apoptosis in various cell types.
- Antibodies against c-Myc epitopes recognize Myc-tagged proteins at amino or carboxy termini.
- Amplification or translocation of c-Myc is found in multiple human cancers, including lung, breast, colon, and Burkitt’s lymphoma.
For Research Use Only. Not for use in diagnostic procedures or resale without authorization.
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