Thermo Fisher Scientific Phosphotyrosine Monoclonal Antibody (pY20)
Phosphotyrosine 단백질 검출용 Mouse Monoclonal Antibody (Clone pY20). Western blot, IHC, ICC, ELISA, IP 등 다양한 응용 가능. Unconjugated 형식으로 제공되며 안정적인 Protein A/G 정제 및 PBS buffer 보존. 연구용으로만 사용 가능.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | Assay-dependent | View 44 publications |
| Immunohistochemistry (IHC) | Assay-dependent | View 2 publications |
| Immunocytochemistry (ICC/IF) | - | View 3 publications |
| ELISA | Assay-dependent | View 1 publication |
| Immunoprecipitation (IP) | Assay-dependent | View 8 publications |
| Miscellaneous PubMed | - | View 19 publications |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Chemical |
| Published Species | Chemical, Human, Mouse |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Clone | pY20 |
| Immunogen | Hybridoma produced from Balb/C mice immunized with phosphotyrosine coupled to carrier protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS |
| Contains | 0.08% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| RRID | AB_2536315 |
Available Formats:
- Biotin ([MA1-12439])
- FITC ([MA1-12441])
- HRP ([MA1-12445])
- Request custom conjugation (Thermo Fisher form)
Target Information
Tyrosine phosphorylation plays a key role in mitogenic signal transduction from transmembrane receptors and oncogene tyrosine kinases. It is a primary mechanism in normal mitogenesis, cell cycle progression, and oncogenic transformation, while also being actively studied in differentiation and gap junction communication.
Antibodies that specifically recognize phosphorylated tyrosine residues are invaluable tools for studying tyrosine-phosphorylated proteins and related biochemical pathways. The FITC conjugate of clone pY20 anti-phosphotyrosine is particularly effective for detecting P-Tyr proteins in immunohistochemical and immunocytochemical protocols, especially where secondary antibody use may interfere with detection.
For Research Use Only.
Not for use in diagnostic procedures or resale without express authorization.
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