Thermo Fisher Scientific DCUN1D1 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

0.3-1 µg/mL

-

Immunohistochemistry (Paraffin) (IHC (P))

5-10 µg/mL

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Goat / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide sequence (RPQIAGTKSTT) corresponding to the C-terminus amino acids of DCUN1D1 (aa 248-259). if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:DCUN1D1,uniProtId:Q96GG9-1,ncbiNodeId:9606,antigenRange:248-259,antigenLength:259,antigenImageFileName:PA5-19284_DCUN1D1_Q96GG9-1_Goat.svg,antigenImageFileNamePDP:PA5-19284_DCUN1D1_Q96GG9-1_Goat_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Ammonium sulfate precipitation

Storage buffer

TBS, pH 7.3, with 0.5% BSA

Contains

0.02% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_10976834

Product Specific Information

This antibody is predicted to react with bovine and mouse based on sequence homology.

This antibody is tested in Peptide ELISA: antibody detection limit dilution 16,000.

Target Information

Part of an E3 ubiquitin ligase complex for neddylation. Promotes neddylation of cullin components of E3 cullin-RING ubiquitin ligase complexes. Acts by binding to cullin-RBX1 complexes in the cytoplasm and promoting their nuclear translocation, enhancing recruitment of E2-NEDD8 (UBE2M-NEDD8) thioester to the complex, and optimizing the orientation of proteins in the complex to allow efficient transfer of NEDD8 from the E2 to the cullin substrates. Involved in the release of inhibitory effets of CAND1 on cullin-RING ligase E3 complex assembly and activity (PubMed:25349211, PubMed:28581483). Acts also as an oncogene facilitating malignant transformation and carcinogenic progression. [UniProt]

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.