Thermo Fisher Scientific TRIM71 Polyclonal Antibody
TRIM71 단백질을 인식하는 염소 유래 폴리클로날 항체. Western blot에 최적화되어 있으며, 마우스에 반응하고 인간, 개, 소, 랫드에도 예측 반응. 0.5 mg/mL 농도의 액상 형태로 제공되며, -20°C에서 보관.
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Applications
Western Blot (WB)
- Tested Dilution: 1–3 µg/mL
- View 1 publication
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Mouse |
| Host / Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide sequence (NKLESTISAVQ) corresponding to the internal amino acids of TRIM71 (aa 397–406) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Ammonium sulfate precipitation |
| Storage Buffer | TBS, pH 7.3, with 0.5% BSA |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_10977393 |
Product Specific Information
- This antibody is predicted to react with bovine, canine, human, and rat based on sequence homology.
- Tested in Peptide ELISA: antibody detection limit dilution 32,000.
Target Information
TRIM71 is the mammalian ortholog to the C. elegans heterochronic protein Lin41, a protein important in postembryonic development and downstream of both Shh and Fgf signaling pathways. Similar to its C. elegans homolog, TRIM71 is a target of the microRNA let-7 and likely plays a role in mammalian development.
Recent studies indicate that TRIM71 functions as an E3 ubiquitin ligase and interacts with Dicer and Argonaute proteins (Ago1, Ago2, Ago4). Overexpression and depletion of TRIM71 lead to inverse changes in Ago2 protein levels, suggesting TRIM71 regulates Ago2 turnover. TRIM71 also cooperates with the pluripotency factor Lin-28 in regulating let-7.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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