Thermo Fisher Scientific HDAC8 Monoclonal Antibody (OTI3F6), TrueMAB
HDAC8 단백질을 인식하는 단일클론 항체로, 인간·마우스·랫트에 반응합니다. Western blot에 최적화되어 있으며, 동결건조 형태로 제공됩니다. PBS 버퍼(8% 트레할로스 포함)에 보관하며, 연구용으로만 사용됩니다.
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Applications
Western Blot (WB)
- Tested Dilution: 1:2,000
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI3F6 |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 1–243 of human HDAC8 produced in E. coli |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | −20 °C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to obtain a final antibody concentration of about 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
In the intact cell, DNA associates with histones and other nuclear proteins to form chromatin. Chromatin remodeling, driven by histone acetylation, plays a crucial role in transcriptional regulation. Acetylation of lysine residues in histone tails induces conformational changes that enhance DNA accessibility to transcription factors, while deacetylation is linked to transcriptional silencing.
HDAC8, a mammalian histone deacetylase isolated from human kidney, shares homology with other HDACs but exhibits distinct tissue distribution. It is localized in the nucleus and involved in tissue development and cancer etiology.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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