Thermo Fisher Scientific EGFR Monoclonal Antibody (UMAB95), UltraMAB
EGFR 단백질을 인식하는 UMAB95 클론의 mouse monoclonal antibody. Western blot, IHC, ICC/IF, ChIP 등 다양한 응용에 적합. Human 및 Dog 반응성. Lyophilized 형태로 제공되며, PBS + 8% trehalose buffer에 보관. 연구용 전용 제품.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100 |
| Immunocytochemistry (ICC/IF) | 1:100 |
| ChIP assay (ChIP) | Assay-Dependent |
| Peptide Array (Array) | Assay-Dependent |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Dog, Human |
| Host/Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | UMAB95 |
| Immunogen | Full length human recombinant protein of human EGFR produced in HEK293T cell |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of about 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
EGFR (epidermal growth factor receptor) is a receptor tyrosine kinase that mediates signaling in response to various growth factors. Overexpression of EGFR is associated with numerous cancers and serves as a target for biological and small molecule therapeutics.
EGFR is encoded by the EGFR gene located on chromosome 7 and belongs to the HER/ERbB family, which includes ERbB2, ERbB3, and ERbB4.
Binding of ligands such as EGF and TGFα induces receptor dimerization and autophosphorylation, activating downstream signaling pathways.
Overexpression is observed in tumors of the head and neck, brain, bladder, stomach, breast, lung, endometrium, cervix, vulva, ovary, esophagus, and squamous cell carcinoma.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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