Thermo Fisher Scientific Cytokeratin 19 Monoclonal Antibody (A53-B/A2.26 (Ks19.1))

Applications

Tested Dilution

Publications

Western Blot (WB)

2 µg/mL

View 6 publications 6 publications

Immunohistochemistry (IHC)

-

View 23 publications 23 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:200

View 9 publications 9 publications

Immunocytochemistry (ICC/IF)

2 µg/mL

View 13 publications 13 publications

Dot blot (DB)

-

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human

Published species

Cat, Human, Mouse, Rhesus monkey, Sheep

Host/Isotype

Mouse / IgG2a, lambda

Class

Monoclonal

Type

Antibody

Clone

A53-B/A2.26 (Ks19.1)

Immunogen

Human breast cancer MCF-7 cells if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:Cytokeratin 19,uniProtId:P08727-1,ncbiNodeId:9606,antigenRange:1-400,antigenLength:400,antigenImageFileName:MA5-12663_Cytokeratin_19_P08727-1_House_mouse.svg,antigenImageFileNamePDP:MA5-12663_Cytokeratin_19_P08727-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.2 mg/mL

Purification

Protein G

Storage buffer

PBS, pH 7.4, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10984317

Product Specific Information

MA5-12663 targets Cytokeratin 19 in IF, IHC (P), and WB applications and shows reactivity with Human samples.

The MA5-12663 immunogen is human breast cancer MCF-7 cells.

Target Information

Cytokeratin 19 is part of a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity, represented in human epithelial tissues by at least 20 different polypeptides. Cytokeratins range in molecular weight from 40-68 kDa and isoelectric pH between 4.9 - 7.8. The individual human cytokeratins are numbered 1 to 20. The various epithelia in the human body usually express cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of maturation or differentiation within an epithelium. Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometric assays. For example, cytokeratin 19 is not expressed in hepatocytes, therefore, it is useful in the identification of liver metastasis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.