Thermo Fisher Scientific Donkey anti-Chicken IgY (H+L) Highly Cross Adsorbed Secondary Antibody, Alexa Fluor 568
닭 IgY(H+L)에 특이적인 당나귀 유래 폴리클로날 2차 항체로 Alexa Fluor 568 형광체 결합. 다양한 면역분석(WB, IHC, ICC, Flow)에 사용 가능. 고도로 교차흡착되어 비특이적 반응 최소화. 높은 형광 안정성과 감도로 정밀한 시각화에 적합.
- 카탈로그번호
- A78950
- 판매단위
- pk
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1 µg/mL |
| Immunohistochemistry (IHC) | Assay-dependent |
| Immunocytochemistry (ICC/IF) | 1–2 µg/mL |
| Flow Cytometry (Flow) | Assay-dependent |
| Peptide Array (Array) | 1:4,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Chicken |
| Host / Isotype | Donkey / IgG |
| Class | Polyclonal |
| Type | Secondary Antibody |
| Immunogen | Purified Chicken IgY, Heavy and Light chains |
| Conjugate | Alexa Fluor™ 568 |
| Excitation / Emission Max | 579 / 603 nm |
| Form | Liquid |
| Concentration | 2 mg/mL |
| Purification | Purified |
| Storage Buffer | PBS, pH 7.5 |
| Contains | 5 mM sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient |
| RRID | AB_2921072 |
| Target | IgY |
| Cross Adsorption | Bovine, goat, guinea pig, Syrian hamster, horse, human, mouse, rabbit, rat, sheep serum |
| Antibody Form | Whole Antibody |
Additional Formats
- Alexa Fluor 488
- Alexa Fluor 555
- Alexa Fluor 594
- Alexa Fluor 647
- Custom conjugation available
Product Specific Information
This donkey anti-chicken IgY (H+L) whole secondary antibody is affinity-purified and highly cross-adsorbed to minimize cross-reactivity with other species’ serum proteins. The cross-adsorption process enhances specificity, resulting in higher sensitivity and reduced background staining. This is particularly beneficial for multiplexing or multicolor staining experiments such as flow cytometry or fluorescence microscopy.
Alexa Fluor™ 568 is a bright orange/red fluorescent dye with excitation suitable for the 568 nm laser line. It exhibits high photostability and pH insensitivity, allowing consistent signal generation across a wide range of conditions. The labeling ratio is typically 2–8 fluorophores per IgG molecule, as indicated in the certificate of analysis.
Usage recommendation:
Before use, briefly centrifuge the conjugate solution and use the supernatant to minimize aggregates. Optimal antibody dilution should be determined empirically; typically, 1–10 µg/mL is suitable for most IHC and flow cytometry applications.
Product is shipped at room temperature.
Target Information
Anti-Chicken secondary antibodies are affinity-purified reagents with well-characterized specificity for chicken immunoglobulins. They are widely used for detection, sorting, and purification of target proteins. Secondary antibodies enhance signal detection through amplification and are available with various labels for different detection systems (e.g., HRP, AP, fluorescence).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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