Thermo Fisher Scientific Donkey anti-Chicken IgY (H+L) Highly Cross Adsorbed Secondary Antibody, Alexa Fluor 488

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Alexa Fluor 488 형광으로 표지된 당나귀 유래 항-닭 IgY (H+L) 2차 항체로, 높은 교차 흡착 처리로 비특이적 반응을 최소화합니다. 면역형광, IHC, Flow Cytometry 등 다양한 응용에 적합하며, 499/520 nm 파장에서 안정적이고 밝은 녹색 형광 신호를 제공합니다.

카탈로그번호: A78948
브랜드: Thermo Fisher Scientific
카테고리: Polyclonal Antibodies
판매단위: pk

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Thermo Fisher Scientific A78948 Donkey anti-Chicken IgY (H+L) Highly Cross Adsorbed Secondary Antibody, Alexa Fluor 488 500 ug pk판매 단위 pk ·

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344,100원VAT 포함 378,510원

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Thermo Fisher Scientific · Thermo Fisher Scientific Donkey anti-Chicken IgY (H+L) Highly Cross Adsorbed Secondary Antibody, Alexa Fluor 488

Applications and Tested Dilution

Application	Tested Dilution
Western Blot (WB)	0.5–1 µg/mL
Immunohistochemistry (IHC)	Assay-dependent
Immunocytochemistry (ICC/IF)	0.25–0.5 µg/mL
Flow Cytometry (Flow)	Assay-dependent
Peptide Array (Array)	1:4,000

Product Specifications

항목	내용
Species Reactivity	Chicken
Host / Isotype	Donkey / IgG
Class	Polyclonal
Type	Secondary Antibody
Immunogen	Purified Chicken IgY, Heavy and Light chains
Conjugate	Alexa Fluor™ 488
Excitation / Emission Max	499 / 520 nm
Form	Liquid
Concentration	2 mg/mL
Purification	Purified
Storage Buffer	PBS, pH 7.5
Contains	5 mM sodium azide
Storage Conditions	4°C, store in dark, DO NOT FREEZE
Shipping Conditions	Ambient
RRID	AB_2921070
Target	IgY
Cross Adsorption	Against bovine, goat, guinea pig, syrian hamster, horse, human, mouse, rabbit, rat and sheep serum
Antibody Form	Whole Antibody

Product Specific Information

This donkey anti-chicken IgY (H+L) whole secondary antibody has been affinity-purified and shows minimal cross-reactivity with serum proteins from other species. Cross-adsorption enhances specificity, resulting in higher sensitivity and lower background staining. This is particularly beneficial for multiplex or multicolor staining experiments, such as flow cytometry or immunofluorescence, where potential cross-reactivity can occur.

Alexa Fluor™ 488 dye provides bright, green fluorescence ideally excited at 488 nm. It is pH-insensitive over a wide range, highly photostable, and suitable for detecting low-abundance targets. Each antibody is typically labeled with 2–8 dye molecules per IgG, as indicated in the certificate of analysis.

Usage Tips:

Centrifuge the conjugate briefly before use and use only the supernatant to minimize aggregates.
Recommended working concentration: 1–10 µg/mL for most applications.
Product ships at room temperature.

Target Information

Anti-Chicken secondary antibodies are affinity-purified antibodies with well-characterized specificity for chicken immunoglobulins. They are widely used for detection, sorting, or purification of chicken IgY. Secondary antibodies enhance assay sensitivity and versatility by enabling multiple detection systems such as HRP, AP, and fluorescence. These antibodies are typically generated by immunizing the host animal with pooled immunoglobulins from the target species and further purified or modified for improved specificity.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.