Thermo Fisher Scientific COL1A1 Polyclonal Antibody
COL1A1 단백질을 인식하는 Rabbit Polyclonal 항체로, WB, IHC, ICC, IP 등 다양한 응용에 사용 가능. Human, Mouse, Rat에 반응하며 고순도 항원 친화 크로마토그래피 정제. 장기 보관 시 -20°C 권장. 연구용으로만 사용.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500–1:3,000 | View 13 publications |
| Immunohistochemistry (IHC) | – | View 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:1,000 | – |
| Immunocytochemistry (ICC/IF) | 1:100–1:1,000 | View 3 publications |
| Immunoprecipitation (IP) | 1:100–1:500 | – |
Product Specifications
| Item | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published Species | Chicken, Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant fragment corresponding to a region within amino acids 219–478 of Human COL1A1 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.09 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7, with 1% BSA, 20% glycerol |
| Contains | 0.025% ProClin 300 |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2547045 |
Product Specific Information
- Recommended positive controls: SK-N-SH, SK-N-AS, NIH3T3
- Predicted reactivity: Mouse (96%), Rat (94%), Xenopus laevis (83%), Dog (98%), Bovine (98%)
- Store as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
The COL1A1 gene encodes the pro-alpha1 chain of type I collagen, a fibril-forming collagen found in most connective tissues, including bone, cornea, dermis, and tendon.
Mutations in COL1A1 are associated with osteogenesis imperfecta (types I–IV), Ehlers-Danlos syndrome (types VIIA and Classical), Caffey Disease, and idiopathic osteoporosis.
Reciprocal translocations between chromosomes 17 and 22 involving COL1A1 and PDGFB genes are linked to dermatofibrosarcoma protuberans due to unregulated growth factor expression.
Two transcripts have been identified due to alternate polyadenylation signals.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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