Thermo Fisher Scientific Catenin, beta (p120) Recombinant Rabbit Monoclonal Antibody (CTNNB1, 2030R)
Thermo Fisher의 CTNNB1, 2030R은 재조합 토끼 단클론 항체로, 인간 베타-카테닌을 특이적으로 인식합니다. WB, IHC, ICC, Flow Cytometry 등 다양한 응용에 적합하며, 높은 순도와 재현성을 제공합니다. 연구용으로만 사용 가능합니다.
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Thermo Fisher Scientific Catenin, beta (p120) Recombinant Rabbit Monoclonal Antibody (CTNNB1, 2030R)
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–2 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1–2 µg/mL |
| Immunocytochemistry (ICC/IF) | 1–2 µg/mL |
| Flow Cytometry (Flow) | 1–2 µg/10⁶ cells |
| Peptide Array (Array) | Assay-dependent |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | CTNNB1, 2030R |
| Immunogen | Recombinant full-length human beta-catenin protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 200 µg/mL |
| Purification | Protein A |
| Storage Buffer | PBS, pH 7.4, with 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Immunohistochemistry (PFA fixed): Incubate antibody for 30 min at room temperature.
- For formalin-fixed tissues: Heat tissue sections in 10 mM Tris with 1 mM EDTA (pH 9.0) for 45 min at 95°C, then cool at room temperature for 20 min.
Target Information
Beta-catenin is an adherens junction (AJ) protein crucial for cell-cell adhesion and epithelial layer maintenance. It interacts with E-cadherin and alpha-catenin to anchor the adhesion complex to the actin cytoskeleton. Beta-catenin also associates with signaling complexes involving APC and microtubules, participating in Wnt/Wingless pathways that regulate morphogenesis and cell proliferation.
Beta-catenin expression is observed in various hair follicle cells and epithelial structures. Abnormal beta-catenin activity has been linked to pilomatrixoma, medulloblastoma, colorectal, ovarian, and other solid tumors. In the nucleus, beta-catenin coactivates Lef/Tcf transcription factors, promoting transcription of genes such as cyclin D and c-myc, contributing to tumorigenesis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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