Takara Edman degradation and protein sequencing: Pfu Aminopeptidase I
Recombinant Pfu Aminopeptidase I from Pyrococcus furiosus expressed in E. coli. Efficiently liberates N-terminal amino acids, optimal activity at high temperature and variable pH with Co2+. Lyophilized form for protein sequencing research use only.
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Takara Edman Degradation and Protein Sequencing: Pfu Aminopeptidase I
Applications
- Liberates the N-terminal amino acids up to X-Pro from proteins and peptides.
Source
Recombinant Escherichia coli encoding the Pyrococcus furiosus aminopeptidase I gene.
Properties
| 항목 | 내용 |
|---|---|
| 분자량 | 37.483 kDa (calculated), 36–37 kDa (SDS-PAGE) |
| 등전점 (pI) | 4.6–4.65 |
| 저해제 | EDTA (completely inhibited at 0.1 mM) |
| 최적 pH | 5.5–6.0 (without Co2+, 90°C) / 5.5–8.0 (with 20 µM Co2+, 90°C) |
| 최적 온도 | 80°C (pH 6.0, without Co2+) / 95°C (pH 6.0, with 20 µM Co2+) |
| 열 안정성 | 65% activity after 4 hours at 90°C (without Co2+ at pH 8.0) |
Definition of Activity
One unit of enzyme activity corresponds to the amount required to hydrolyze 1 µmol of Leucine-p-nitroanilide at 75°C, pH 8.0 in one minute.
Activities
| 조건 | 활성 |
|---|---|
| Without Co2+ | >16 U/mg protein |
| With 20 µM Co2+ | >80 U/mg protein |
Form
Lyophilized.
Additional Product Information
Please refer to the product’s Certificate of Analysis for information about storage conditions, product components, and technical specifications.
See the Kit Components List to determine kit contents.
Certificates of Analysis and Kit Components Lists are available under the Documents tab.
Takara Bio USA, Inc.
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FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES.
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