Takara Edman degradation and protein sequencing: Pfu Aminopeptidase I

More Information

Applications

• Liberates the N-terminal amino acids up to X-Pro from proteins and peptides

Source

Recombinant Escherichia coli encoding the Pyrococcus furiosus aminopeptidase I gene

Properties

• Molecular weight: 37.483 kDa (calculated), 36–37 kDa (SDS-PAGE)
• Isoelectric point: 4.6–4.65
• Inhibitor: EDTA (completely inhibited at 0.1 mM)
• Optimum pH: 5.5–6.0 (in the absence of Co2+ at 90°C)

                   5.5–8.0 (in the presence of 20 µM Co2+ at 90°C)

• Optimum temperature: 80°C (pH 6.0 in the absence of Co2+) 95°C (pH 6.0 in the presence of 20 µM Co2+)
• Thermal stability: 65% activity after 4 hours at 90°C (without Co2+ at pH 8.0)

Definition of activity

One unit of enzyme activity corresponds to the amount required to hydrolyze 1 µmol of Leucine-p-nitroanilide at 75°C, pH 8.0 in one minute.

Activities

• >16 U/mg protein (without Co2+)
• >80 U/mg protein (in the presence of 20 µM Co2+)

Form

Lyophilized

Additional product information

Please see the product`s Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.

Takara Bio USA, Inc.
United States/Canada: +1.800.662.2566 • Asia Pacific: +1.650.919.7300 • Europe: +33.(0)1.3904.6880 • Japan: +81.(0)77.565.6999
FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES. © 2023 Takara Bio Inc. All Rights Reserved. All trademarks are the property of Takara Bio Inc. or its affiliate(s) in the U.S. and/or other countries or their respective owners. Certain trademarks may not be registered in all jurisdictions. Additional product, intellectual property, and restricted use information is available at takarabio.com.