Takara Edman degradation and protein sequencing: Pfu Aminopeptidase I
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카탈로그 번호 | CAS 번호 | 설명 | 상태 | 단위 | 판매가 | 할인가 | 가격(VAT포함) | 수량 / 장바구니 / 찜 |
7336 | - | Takara 7336 Pfu Aminopeptidase I, 0.5 mg pk | 재고문의 | pk | 189,000원 | - | 207,900원 |
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More Information
Applications
- Liberates the N-terminal amino acids up to X-Pro from proteins and peptides
Source
Recombinant Escherichia coli encoding the Pyrococcus furiosus aminopeptidase I gene
Properties
- Molecular weight: 37.483 kDa (calculated), 36–37 kDa (SDS-PAGE)
- Isoelectric point: 4.6–4.65
- Inhibitor: EDTA (completely inhibited at 0.1 mM)
- Optimum pH: 5.5–6.0 (in the absence of Co2+ at 90°C)
5.5–8.0 (in the presence of 20 µM Co2+ at 90°C)
- Optimum temperature: 80°C (pH 6.0 in the absence of Co2+) 95°C (pH 6.0 in the presence of 20 µM Co2+)
- Thermal stability: 65% activity after 4 hours at 90°C (without Co2+ at pH 8.0)
Definition of activity
One unit of enzyme activity corresponds to the amount required to hydrolyze 1 µmol of Leucine-p-nitroanilide at 75°C, pH 8.0 in one minute.
Activities
- >16 U/mg protein (without Co2+)
- >80 U/mg protein (in the presence of 20 µM Co2+)
Form
Lyophilized
Additional product information
Please see the product`s Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
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