Thermo Fisher Scientific PAPD5 Polyclonal Antibody
Rabbit polyclonal antibody against human PAPD5, validated for IHC-P. Recognizes recombinant human PAPD5 protein. Supplied in PBS with 40% glycerol, 0.02% sodium azide. For research use only.
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Applications
Tested Application
- Immunohistochemistry (Paraffin) (IHC-P)
Tested Dilution
- 1:50–1:200
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein corresponding to Human PAPD5. Recombinant protein control fragment: Product #RP-98641 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 40% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | Store at 4°C short term. For long-term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2645196 |
Product Specific Information
Immunogen sequence:
QSSSSDVDSD ATPCKTPKQL LCRPSTGNRV GSQDVSLESS QAVGKMQSTQ TTNTSNSTNK SQHGSARLFR SSSKGFQGTT QTSH
Sequence identity (orthologs):
- Mouse: 90%
- Rat: 92%
Target Information
PAPD5 is a terminal nucleotidyltransferase that preferentially transfers ATP and GTP to RNA 3′ poly(A) tails, creating heterogeneous tails that stabilize mRNAs by protecting them from deadenylation. It functions as a catalytic subunit of a TRAMP-like complex with poly(A) RNA polymerase activity involved in post-transcriptional quality control. PAPD5-mediated short oligo(A) polyadenylation is required for exosome-mediated RNA degradation. It also participates in cytoplasmic polyadenylation of mRNAs involved in carbohydrate metabolism (e.g., SLC2A1/GLUT1) and in histone mRNA degradation via terminal uridylation. Additionally, it may contribute to sister chromatid cohesion and mediates 3′ adenylation of microRNA MIR21 and H/ACA box snoRNAs, influencing their stability and maturation.
[UniProt]
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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