Thermo Fisher Scientific PPP4R3 Alpha Polyclonal Antibody
PPP4R3 Alpha 단백질을 인식하는 Thermo Fisher Scientific의 Rabbit Polyclonal Antibody. Western blot, IHC, IP 등 다양한 응용에 적합. 항원 친화 크로마토그래피로 정제된 액상 제품이며, 인산화 H2AFX 탈인산화 연구에 활용 가능.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000–1:10,000 |
| Immunohistochemistry (IHC) | 1:50–1:500 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:500 |
| Immunoprecipitation (IP) | 2–5 µg/mg lysate |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Region between residue 770 and the C-terminus (residue 820) of Human Protein Phosphatase 4, Regulatory Subunit 3 Alpha (KIAA2010) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.20 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | TBS, pH 7.0–8.0, with 0.1% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
- Recommended shelf life: 1 year from date of receipt
- Application Note: For IHC, epitope retrieval with Tris-EDTA pH 9.0 is recommended for FFPE tissue sections.
Target Information
Regulatory subunit of serine/threonine-protein phosphatase 4. May regulate the activity of PPP4C at centrosomal microtubule organizing centers. The PPP4C–PPP4R2–PPP4R3A PP4 complex specifically dephosphorylates H2AFX phosphorylated on Ser-140 (gamma-H2AFX) generated during DNA replication and required for DNA double-strand break repair.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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