Thermo Fisher Scientific FADD Recombinant Rabbit Monoclonal Antibody (10L6M0)
FADD 단백질을 표적으로 하는 Thermo Fisher Scientific의 재조합 토끼 단일클론 항체. WB, IHC, ELISA 등 다양한 응용에 적합하며 높은 특이성과 재현성을 제공. HEK293 발현 시스템 기반, 액상 형태로 -20°C 보관.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| ELISA | 1 µg/mL |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | 10L6M0 |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 1–205 of mouse FADD (NP_0343051) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 0.05% BSA, 50% glycerol |
| Contains | 0.05% ProClin 300 |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2897950 |
Product Specific Information
- Positive Samples: NIH/3T3, Mouse brain, Mouse thymus
- Immunogen Sequence:
MDPFLVLLHS LSGSLSGNDL MELKFLCRER VSKRKLERVQ SGLDLFTVLL EQNDLERGHT GLLRELLASL RRHDLLQRLD DFEAGTATAA PPGEADLQVA FDIVCDNVGR DWKRLARELK VSEAKMDGIE EKYPRSLSER VRESLKVWKN AEKKNASVAG LVKALRTCRL NLVADLVEEA QESVSKSENM SPVLRDSTVS SSETP
Target Information
The FADD protein is an adaptor molecule that interacts with various cell surface receptors to mediate apoptotic signaling. Through its C-terminal death domain, it can be recruited by TNFRSF6/Fas-receptor, TNFRSF25, and TNFSF10/TRAIL-receptor, participating in death signaling initiated by these receptors. This interaction exposes the N-terminal effector domain, enabling recruitment of caspase-8 and activation of the cysteine protease cascade. Knockout studies in mice highlight its crucial role in early T cell development.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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