Thermo Fisher Scientific Phospho-KAP-1 (Ser824) Polyclonal Antibody

Applications

Western Blot (WB)

• Tested Dilution: 1:500–1:2,500

Immunoprecipitation (IP)

• Tested Dilution: Assay-dependent

Product Specifications

Product Specific Information

• Recommended shelf life: 1 year from date of receipt
• Based on 100% sequence identity, this antibody is predicted to react with Rat

Target Information

The KRAB (Kruppel-associated box) domain, approximately 45 amino acids in length, is a transcriptional repression domain found in numerous transcription factors. Over 220 KRAB-zinc finger protein (KRAB-ZFP) genes have been identified in the human genome. These proteins repress transcription via interactions with KAP-1 (KRAB-associated protein 1), an 835 amino acid polypeptide containing a RING finger, B boxes, and a PHD finger.
KAP-1 forms complexes with KRAB-domain transcription factors to enhance repression efficiency and interacts directly with HP1 (heterochromatin protein 1) and KRAZ1 (Kruppel-associated box-containing zinc finger protein 1). KAP-1 targets KRAZ1 to centromeric heterochromatin foci containing HP1alpha, contributing to transcriptional repression regulation. Mutant KAP-1 unable to bind HP1 but retaining KRAB binding leads to random KRAZ1 distribution and strong transcriptional activation.

For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.