Thermo Fisher Scientific SHMT1 Polyclonal Antibody
SHMT1 단백질을 인식하는 Rabbit Polyclonal 항체로, Western blot, IHC, ICC, ELISA 등 다양한 응용 가능. Human, Mouse, Rat에 반응하며, Protein A 정제 및 고농도(1 mg/mL) 액상 형태로 제공. 연구용으로만 사용.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:300 |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:200–1:400 |
| Immunohistochemistry (Frozen) (IHC-F) | 1:100–1:500 |
| Immunocytochemistry / Immunofluorescence (ICC/IF) | 1:100–1:500 |
| ELISA | 1:500–1:1,000 |
Product Specifications
| Item | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | KLH-conjugated synthetic peptide derived from human SHMT1, amino acids 301–400 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | TBS, pH 7.4, with 50% glycerol, 1% BSA |
| Contains | 0.03% ProClin 300 |
| Storage Conditions | –20 °C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
The SHMT1 gene encodes the cytosolic form of serine hydroxymethyltransferase, a pyridoxal phosphate-containing enzyme that catalyzes the reversible conversion of serine and tetrahydrofolate to glycine and 5,10-methylene tetrahydrofolate. This reaction provides one-carbon units for the synthesis of methionine, thymidylate, and purines in the cytoplasm. The gene is located within the Smith-Magenis syndrome region on chromosome 17. Alternative splicing results in two transcript variants encoding different isoforms, with additional variants of uncertain biological validity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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