Thermo Fisher Scientific Phospho-PKC alpha (Thr638) Polyclonal Antibody

Applications and Tested Dilution

Product Specifications

Product Specific Information

It is not recommended to aliquot this antibody.

Target Information

The PKC family of serine/threonine kinases, including PRKCA (PKC alpha), is activated intracellularly by signal transduction pathways. In humans, at least 12 different PKC polypeptides have been identified. These isoforms differ in primary structure, tissue distribution, subcellular localization, mode of action in vitro, response to extracellular signals, and substrate specificity.

PKC alpha, beta I, beta II, and gamma form the conventional family; their activities are Ca²⁺- and phospholipid-dependent. Protein kinase C (PKC) can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are involved in diverse cellular signaling pathways. They also serve as major receptors for phorbol esters, a class of tumor promoters.

Each PKC family member has a specific expression profile and distinct cellular role. The PKC alpha kinase is implicated in processes such as cell adhesion, transformation, cell cycle checkpoint regulation, and cell volume control. Knockout studies in mice suggest it is a key regulator of cardiac contractility and Ca²⁺ handling in myocytes.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.