Thermo Fisher Scientific MAP2K3 Monoclonal Antibody (OTI5D3), TrueMAB

Applications and Tested Dilutions

• Western Blot (WB): 1:200–1:2,000
• Immunohistochemistry (Paraffin) (IHC-P): 1:150
• Immunocytochemistry (ICC/IF): 1:100

Product Specifications

Product Specific Information

For reconstitution, add 100 µL of distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).

Target Information

MAP2K3 (MEK3) is a dual-specificity protein kinase involved in cellular signal transduction pathways and is necessary for glucose transporter expression. It phosphorylates and activates MAPK14/p38-MAPK and can be activated by insulin.
RAS oncogene expression leads to constitutive activation of MAPK14 through MAP2K3 activation, contributing to oncogenic transformation.
Inhibition of MAP2K3 is implicated in the pathogenesis of Yersinia pseudotuberculosis.
Multiple alternatively spliced transcript variants encoding distinct isoforms have been reported for MEK3.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.