Thermo Fisher Scientific CDC123 Monoclonal Antibody (OTI1E12), TrueMAB
CDC123 단백질을 인식하는 Mouse IgG2a 단클론 항체로, Western blot과 IHC(Paraffin)에 적합. HEK293T에서 발현된 full-length human CDC123을 면역원으로 사용. Lyophilized 형태, PBS buffer에 trehalose 포함, 연구용으로만 사용.
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Applications
Western Blot (WB)
- Tested Dilution: 1:2,000
Immunohistochemistry (Paraffin) (IHC (P))
- Tested Dilution: 1:150
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI1E12 |
| Immunogen | Full length human recombinant protein of human CDC123 produced in HEK293T cell |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
The BCKDHA gene encodes the E1-alpha subunit of the branched-chain alpha-keto acid dehydrogenase complex (BCKD).
This complex consists of three catalytic components:
- A heterotetrameric (α₂-β₂) branched-chain α-keto acid decarboxylase (E1)
- A homo-24-meric dihydrolipoyl transacylase
- A homodimeric dihydrolipoamide dehydrogenase
E1 is a thiamine pyrophosphate (TPP)-dependent enzyme, catalyzing the irreversible first step in branched-chain amino acid oxidation.
The BCKDHB gene encodes the beta subunit of E1, and the complex also includes two regulatory enzymes: a kinase and a phosphorylase.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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