Thermo Fisher Scientific eIF3e Recombinant Rabbit Monoclonal Antibody (ARC1997)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

-

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200

-

ELISA (ELISA)

1 µg/mL

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

ARC1997

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 210-350 of human eIF3e (P60228) if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:eIF3e,uniProtId:P60228-1,ncbiNodeId:9606,antigenRange:210-350,antigenLength:445,antigenImageFileName:MA5-38067_eIF3e_P60228-1_Rabbit.svg,antigenImageFileNamePDP:MA5-38067_eIF3e_P60228-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol, 0.05% BSA

Contains

0.02% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2897985

Product Specific Information

Positive Samples: HeLa, 293T, Jurkat, Mouse lung, Mouse testis, Rat lung, Rat testis

Immunogen sequence: QRTWLIHWSL FVFFNHPKGR DNIIDLFLYQ PQYLNAIQTM CPHILRYLTT AVITNKDVRK RRQVLKDLVK VIQQESYTYK DPITEFVECL YVNFDFDGAQ KKLRECESVL VNDFFLVACL EDFIENARLF IFETFCRIHQ C

Target Information

Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. Required for nonsense-mediated mRNA decay (NMD); may act in conjunction with UPF2 to divert mRNAs from translation to the NMD pathway. May interact with MCM7 and EPAS1 and regulate the proteasome-mediated degradation of these proteins.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.