Thermo Fisher Scientific Bac-to-Bac Baculovirus Expression System

Gibco™ Bac-to-Bac™ Baculovirus Expression System

The Bac-to-Bac™ Baculovirus Expression System enables efficient production of recombinant baculovirus for expression testing in insect cells.
This system relies on generation of recombinant baculovirus by site-specific transposition in E. coli rather than homologous recombination in insect cells.

주요 특징

• Time-saving expression bacmid
  The expression cassette of the pFastBac vector recombines with the parent bacmid in DH10Bac E. coli Competent Cells to form an expression bacmid, which is then transfected into insect cells for recombinant baculovirus production.

• Easy colony screening
  The parent bacmid in DH10Bac E. coli contains a segment of the lacZa gene. Upon transposition, disruption of lacZa allows blue/white selection for easy identification of recombinant colonies.

• High transfection efficiency
  Includes the next-generation ExpiFectamine Sf Transfection Reagent for efficient DNA transfection in insect cells using fast, flexible protocols.
  Find out more about this reagent ›

• Flexibility
  The pFastBac vector features a large multiple cloning site (MCS) for simplified cloning.
  Additional vector options:

  • pFastBac HT vector: for histidine-tagged recombinant proteins
  • pFastBac Dual vector: for dual protein expression using p10 and polyhedrin promoters

• High protein expression
  The pFastBac vector uses the strong polyhedrin promoter to generate high expression levels in various insect cell lines such as Sf9, Sf21, and High Five™ cells.

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